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1. Oct 08,  · Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette can easily be altered to make different proteins.

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2. Jun 08,  · An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding sequences so that the same cassette .

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3. Use of the mET-1 enhancer-promoter and a genomic IL clone as a strategy to obtain high-level, EC-specific transgene expression. We used the potentially therapeutic26 rabbit interleukin (IL) cDNA and gene as reporters in experiments aimed at developing improved expression cassettes. Our initial hypotheses were that the native mET-1 enhancer-promoter would Cited by:

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5. Expression Cassette. Expression cassettes encoding engineered siRNA (directed to specific mRNAs) can be efficiently introduced into the host cell by use of viral vector systems (e.g., retroviral, adenoviral, and lentiviral vectors) resulting in longterm silencing of target gene expression, both in cultured cells and in animal models–32 Expression vectors for the .

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